This is a guide to the modification and crosslinking of biomolecules for use in research, diagnostics and therapeutics. It provides detailed information on the chemistry, reagent systems, and practical applications for creating labelled or conjugate molecules. It also describes a range of reactions with details on commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids and synthetic polymers. This text is intended to help readers form unique complexes that can be used for detecting, quantifying and targeting important analytes. It is aimed at researchers in biochemistry, molecular biology, immunology, pharmacology, cell biology and microbiology.
- Hardback | 721 pages
- 181.36 x 261.37 x 41.66mm | 1,542.21g
- 01 Jan 1996
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
Table of contents
Part 1 Bioconjugate chemistry: functional targets; the chemistry of reactive groups. Part 2 Bioconjugate reagents: zero-length cross-linkers; homobifunctional cross-linkers; heterobifunctional cross-linkers; trifunctional cross-linkers; cleavable reagent systems; tags and probes. Part 3 Bioconjugate applications: preparation of hapten-carrier immunogen conjugates; antibody modification and conjugation; immunotoxin conjugation techniques; preparation of liposome conjugates and derivatives; avidin-biotin systems; preparation of colloidal gold labelled proteins; modification with synthetic polymers; enzyme modification and conjugation; nucleic acid and oligonucleotide modification and conjugation.
"This is a well-organized and well-written book; it is readable and comprehensible to the novice, but its content and stylistic approach seem sufficiently sophisticated to appeal to active, knowledgeable workers in the field. It is an impressive compilation of useful theoretical and practical information that is not readily available elsewhere in a single volume."--Joseph G. Cannon in JOURNAL OF MEDICINAL CHEMISTRY"The presentation throughout is that of a laboratory manual. Well-illustrated descriptions of a particular chemical reaction are followed by a detailed protocol and list of references extending into 1994. The coverage of reagents and methods is comprehensive, and sources are given for commercially available compounds."--NATURE