Baculovirus Expression Vectors

Baculovirus Expression Vectors : A Laboratory Manual

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Description

This laboratory manual simplifies selection of the most appropriate baculovirus vector design for a given problem. It then describes each step of the implementation process - from vector construction to large-scale protein production. The book provides an understanding of how the vectors work, a biological overview of cells, viruses, plasmids, and promoters, guidelines for choosing optimum vectors, protocols for growing insect cells and recombinant viruses, methods for analysing protein products and scaling up protein production, techniques for producing proteins in insect larvae, and easy to use maps charting available expression vectors.show more

Product details

  • Spiral bound | 360 pages
  • 236.2 x 276.9 x 25.4mm | 793.8g
  • Oxford University Press Inc
  • New York, United States
  • English
  • Revised ed.
  • halftones, line figures, tables
  • 0195091310
  • 9780195091311

Review quote

"A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this area who wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, et al. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work." --Cell"Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of the questions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of anyone using the system at present, or who requires an overview prior to taking the plunge." --Tibtechshow more