Bacterial Persistence
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Bacterial Persistence : Methods and Protocols

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Description

This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia coli cultures, protocols for the determination of the persister subpopulation in Candida albicans, quantitative measurements of Type I and Type II persisters using ScanLag, to in vitro and in vivo models for the study of the intracellular activity of antibiotics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols brings together the most respected researchers in bacterial persistence whose studies will remain vital to understanding this field for many years to come.show more

Product details

  • Hardback | 244 pages
  • 178 x 254 x 19.56mm | 744g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 1st ed. 2016
  • 4 Tables, black and white; 30 Illustrations, color; 22 Illustrations, black and white; XI, 244 p. 52 illus., 30 illus. in color.
  • 1493928538
  • 9781493928538
  • 1,585,497

About Jan Michiels

Jan Michiels Centre of Microbial and Plant Genetics Department of Microbial and Molecular Systems, KU Leuven - University of Leuven Kasteelpark Arenberg 20 box 2460, B-3001 Heverlee Phone: +32 16 32 16 31 (secretariat) or +32 16 32 96 84 (office) Fax.: +32 16 32 19 63 Email: jan.michiels@biw.kuleuven.be Maarten Fauvart Centre of Microbial and Plant Genetics Department of Microbial and Molecular Systems, KU Leuven - University of Leuven Kasteelpark Arenberg 20 box 2460, B-3001 Heverlee Tel: +32 16 32 16 31 (secretariat) or +32 16 32 96 80 (office); Fax: +32 16 32 19 63 Email: maarten.fauvart@biw.kuleuven.beshow more

Back cover copy

This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia coli cultures, protocols for the determination of the persister subpopulation in Candida albicans, quantitative measurements of Type I and Type II persisters using ScanLag, to in vitro and in vivo models for the study of the intracellular activity of antibiotics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols brings together the most respected researchers in bacterial persistence whose studies will remain vital to understanding this field for many years to come.show more

Table of contents

Part I: Introduction1. A Historical Perspective on Bacterial PersistenceNatalie Verstraeten, Wouter Knapen, Maarten Fauvart, Jan MichielsPart II: Quantification of Persistence2. Persisters-Methods for Isolation and Identifying Contributing Factors: A ReviewSarah E. Rowe, Brian P. Conlon, Iris Keren, and Kim Lewis3. A General Method for Measuring Persister Levels in Escherichia Coli CulturesNiilo Kaldalu, Arvi Joers, Henri Ingelman, and Tanel Tenson4. Optimized Method for Measuring Persistence in Escherichia cCli with Improved ReproducibilityF. Goormaghtigh and L. Van Melderen5. A Microplate-Based System as In Vitro Model of Biofilm Growth and QuantificationIlse Vandecandelaere, Heleen Van Acker, and Tom Coenye6. Protocol for Determination of the Persister Subpopulation in Candida Albicans BiofilmsKatrijn De Brucker, Kaat De Cremer, Bruno P.A. Cammue, and Karin ThevissenPart III: Single Cell Analysis of Persister Cells7. Quantitative Measurements of Type I and Type II Persisters Using ScanLagIrit Levin-Reisman and Nathalie Q. Balaban8. Analyzing Persister Physiology with Fluorescence-Activated Cell SortingMehmet A. Orman, Theresa C. Henry, Christina J. DeCoste, and Mark P. Brynildsen9. Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet ArrayRyota Iino, Shouichi Sakakihara, Yoshimi Matsumoto, and Kunihiko NishinoPart IV: Identification of Persister Mutants and Genes10. A Whole-Cell Based High-Throughput Screening Method to Identify Molecules Targeting Pseudomonas Aeruginosa Persister CellsVeerle Liebens, Valerie Defraine, and Maarten Fauvart11. Functional Analysis of the Role of Toxin-Antitoxin (TA) Loci in Bacterial PersistenceAaron T. Butt and Richard W. Titball12. Experimental Evolution of Escherichia Coli Persister Levels Using Cyclic Antibiotic TreatmentsBram Van den Bergh, Joran E. Michiels, and Jan MichielsPart V: Cellular and Animal Model Systems for Studying Persistence13. In Vitro Models for the Study of the Intracellular Activity of AntibioticsJulien M. Buyck, Sandrine Lemaire, Cristina Seral, Ahalieyah Anantharajah, Frederic Peyrusson, Paul M. Tulkens, and F. Van Bambeke14. A Murine Model for Escherichia Coli Urinary Tract InfectionThomas J. Hannan and David A. Hunstad15. Analysis of Macrophage-Induced Salmonella PersistersRobert A. Fisher, Angela M. Cheverton, and Sophie Helaine16. Population Dynamics Analysis of Ciprofloxacin-Persistent S. Typhimurium Cells in a Mouse Model for Salmonella DiarrheaPatrick Kaiser, Roland R. Regoes, and Wolf-Dietrich HardtPart VI: Mathematical Modeling of Persistence17. Computational Methods to Model PersistenceAlexandra Vandervelde, Remy Loris, Jan Danckaert, and Lendert Gelensshow more