B Cell Protocols

B Cell Protocols

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B-lymphocyte development and function remains an exciting area of research for those interested in the physiology and pathology of the immune system in higher animals. While recent advances in genetics and cellular and molecular biology have provided a large spectrum of powerful new experimental tools in this field, it is both time consuming and often very difficult for a student or just any bench-side worker to identify a reliable experimental protocol in the ocean of the literature. The aim of B Cell Protocols is to provide a collection of diverse protocols ranging from the latest inventions and applications to some classic, but still frequently used methods in B-cell biology. The authors of the various chapters are all highly qualified scientists who are either the inventors or expert users of these methods. Their extensive experience in mastering a particular method provides not only the step-by-step details of a reproducible protocol, but also useful troubleshooting tips that readers will appreciate in their daily work. We hope that this book will be helpful for both beginning and experienced researchers in the field in designing or modifying an experimental approach, and exploring a biological question from multiple angles.
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Product details

  • Hardback | 292 pages
  • 157.5 x 233.7 x 22.9mm | 612.36g
  • Totowa, NJ, United States
  • English
  • 2004 ed.
  • XII, 292 p.
  • 1588293475
  • 9781588293473

Back cover copy

Recent advances in genetics, synergized by many exciting developments in cellular and molecular biology, have provided powerful new experimental tools for the study of not only B-lymphocyte development and function, but also the physiology and pathology of the immune system in higher animals. In B Cell Protocols, highly qualified investigators who are either the inventors or expert users of their methods provide a diverse collection of protocols that range from the latest inventions and applications to classic, but still frequently used, methods in B cell biology. The protocols presented follow the successful Methods in Molecular Biology(TM) series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, B Cell Protocols offers both beginning and experienced researchers alike highly effective tools for exploring B-lymphocyte development and function in higher animals, as well as critical information on how best to design or modify an experimental approach that will prove productive in their own research.
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Table of contents

Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen
Richard R. Hardy and Susan A. Shinton

Characterization of B-Cell Maturation in the Peripheral Immune System
Rita Carsetti

Identification of B-Cell Subsets: An Exposition of 11-Color (Hi-D) FACS Methods
James W. Tung, David R. Parks, Wayne A. Moore, Leonard A. Herzenberg, and Leonore A. Herzenberg

Analysis of B-Cell Life-Span and Homeostasis
Irmgard Foerster

Differentiation of B Lymphocytes From Hematopoietic Stem Cells
Paulo Vieira and Ana Cumano

Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation System
Faith M. Young, Carl A. Pinkert, and Andrea Bottaro

Conditional Gene Mutagenesis in B-Lineage Cells
Stefano Casola

Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells
Kai-Michael Toellner, Mahmood Khan, and Daniel Man-Yuen Sze

T-Cell-Dependent Immune Responses, Germinal Center Development, and the Anaysis of V-Gene Sequences
Claudia Berek

Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors
Kuo-I Lin and Kathryn Calame

Immunoglobulin Class Switching: In Vitro Induction and Analysis
Sven Kracker and Andreas Radbruch

In Vitro and In Vivo Assays of B-Lymphocyte Migration
Chantal Moratz and John H. Kehrl

Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo
Louise J. McHeyzer-Williams and Michael G. McHeyzer-Williams

B-Cell Signal Transduction: Tyrosine Phosphorylation, Kinase Activity, and Calcium Mobilization
Tilman Brummer, Winfried Elis, Michael Reth, and Michael Huber

Isolation of Lipid Rafts From B Lymphocytes
Anu Cherukuri, Shiang-Jong Tzeng, Arun Gidwani, Hae Won Sohn, Pavel Tolar, Michelle D. Snyder, and Susan K. Pierce

Molecular Single-Cell PCRAnalysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells
Ralf Kuppers

Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice
Helen Ferry and Richard J. Cornall

Analysis of B-Cell Signaling Using DT40 B-Cell Line
Tomoharu Yasuda and Tadashi Yamamoto

B-Cell Development and Pre-B-1 Cell Plasticity In Vitro
Antonius G. Rolink

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