Autoradiography and Correlative Imaging

Autoradiography and Correlative Imaging

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This text is an up-to-date, comprehensive guide for using radioactivity to localize molecules in cells, tissues and whole bodies. It provides historical discussions as well as useful protocols.
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Product details

  • Hardback | 566 pages
  • 156 x 235 x 31mm | 976g
  • Academic Press Inc
  • San Diego, United States
  • English
  • subject index
  • 0126754500
  • 9780126754506

Table of contents

Part 1 Foundations: autoradiography in biomedical research, W.E. Stumpf and C. Pilgrim; history and development of autoradiography, B. Maurer-Schultz and I.D. Bassukas; history of nuclear particle recording, E.J. Hahn; photographic emulsions and processing, D.C. Bundy; qualitative resolution in light microscopic autoradiography, G.E. Duncan; artifacts in autoradiography, W.E. Stumpf and C. Pilgrim. Part 2 "In vivo" autoradiography: localization of steroid receptor ligands with thaw-mount and dry-mount autoradiography, W.E. Stumpf; localization of specific high affinity binding sites (receptors) for ecdysteroids in invertebrates, H.-J. Bidmon and W.E. Stumpf; autoradiographic "in vivo" and "in vitro" studies with different radiolabelled estrogens, H.-J. Rohle et al; rapid high resolution steroid autoradiography, P.J. Shugrue and W.E. Stumpf; "in vivo" and "in vitro" autoradiographic analysis of specific hormone binding sites in rat hippocampus, L. Jennes; distribution of [3H] alendronate in rat femora as examined by reflected polarized light microscopy, W.A. Grasser and M. Sato; whole-body autoradiography - applications in radiopharmaceutical and drug research, P. Som et al. Part 3 "In vitro" receptor autoradiography: "in vitro" labelling autoradiography - development and perspectives, M.J. Kuhar; autoradiographic visualization of neurotransmitter binding sites and mRNAs in human brain, J.M. Palacios et al; mismatches between receptor and transmitter localizations in the opiate system - implications for nonsynaptic opioid actions, M. Herkenhaum; autoradiographic localization of nonreceptor proteins in brain, N. Appel and E.B. De Souza; correlative imaging of transmitter receptor distributions in human cortex, K. Zilles and A. Schleicher; axonal transport of receptors - emphasis on alpha 2 adrenergic binding sites, M.A. Zarbin et al. Part 4 "In situ" hybridization autoradiography: measurement of gene expression in individual neurons by "in situ" hybridization, D.E. Millhorn et al; detection of mRNA in neurons identified by projector or neurochemical content - "in situ" hybridization combined with retrograde tract-tracing immunohistochemistry, D.A. Bayliss et al; developmental transcience of neuropeptide gene expression in rat brainstem as determined by "in situ" hybridization, K.B. Seroogy et al; "in situ" hybridization - a sensitive measure of activity-dependent changes in neuronal gene expression, C. Gall et al. Part 5 Autoradiographic imaging of brain activity. Part 6 Correlative imaging. (Part contents).
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