Autoimmunity : Methods and Protocols

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This collection of readily reproducible molecular techniques and related in vitro/in vivo model systems can be used to explore the causes of autoimmunity, as well as how best it may be regulated. There are methods to assess immunological and biochemical pathways relevant for pathogenesis and to establish and assess a variety of autoimmune diseases, including arthritis, lupus, diabetes, multiple sclerosis, myocarditis, thyroiditis, scleroderma, uveitis, and more

Product details

  • Hardback | 448 pages
  • 158 x 232 x 30mm | 861.84g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 66 black & white illustrations, 5 colour illustrations
  • 1588292312
  • 9781588292315

Back cover copy

Autoimmunity-which affects up to 10% of the world's population-can damage nearly every tissue or cell type of the human body with greatly debilitating or fatal results. In Autoimmunity: Methods and Protocols, expert medical researchers describe in step-by-step detail molecular techniques and in vitro/in vivo model systems for discovering the causes of autoimmunity, as well as how best it may be regulated. The methods to assess the immunological and biochemical pathways relevant for pathogenesis include identifying susceptibility genes, intercellular signaling via cytokines, intracellular signaling through the T-cell receptor, signal processing via protein kinases, identification and enumeration of antigen-specific T cells and autoantibodies, and the dysregulation of apoptosis and its role in modification of self-antigens. Additional protocols serve to establish the presence of and assess inflammatory arthritis, systemic lupus erythematosus, insulin-dependent diabetes mellitus, multiple sclerosis, myocarditis, thyroiditis, scleroderma, uveitis, and vitiligo. These methods can be used to assess the genetic, immunological, and biochemical parameters underlying spontaneous or exogenous antigen-induced diseases. The protocols follow the successful Methods in Molecular Medicine series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Innovative and easy to follow, Autoimmunity: Methods and Protocols offers today's investigators a cutting-edge collection of readily reproducible methods for establishing and interrogating human and animal models of autoimmune more

Table of contents

Pathogenesis and Spectrum of Autoimmunity Andras Perl Part I. Models of Pathogenesis in Human Autoimmune Disorders Mapping the Systemic Lupus Erythematosus Susceptibility Genes Swapan K. Nath, Jennifer A. Kelly, John B. Harley, and R. Hal Scofield T-Cell Signaling Abnormalities in Human Systemic Lupus Erythematosus Madhusoodana P. Nambiar, Sandeep Krishnan, and George C. Tsokos TCR z-Chain Abnormalities in Human Systemic Lupus Erythematosus Madhusoodana P. Nambiar, Sandeep Krishnan, Vishal G. Warke, and George C. Tsokos Protein Kinase A and Signal Transduction in T Lymphocytes: Biochemical and Molecular Methods Islam U. Khan and Gary M. Kammer Apoptosis and Mitochondrial Dysfunction in Lymphocytes of Patients With Systemic Lupus Erythematosus Andras Perl, Gyorgy Nagy, Peter Gergely, Ferenc Puskas, Yueming Qian, and Katalin Banki Methods for Inducing Apoptosis Kathryn M. Roberts, Antony Rosen, and Livia A. Casciola-Rosen Measurement of Cytokines in Autoimmune Disease Kyriakos A. Kirou, Christina Lee, and Mary K. Crow Evaluation of Autoimmunity to Transaldolase in Multiple Sclerosis Brian Niland and Andras Perl Part II. Animal Models of Autoimmunity Animal Models for Autoimmune Myocarditis and Autoimmune Thyroiditis Daniela Cihakova, Rajni B. Sharma, DeLisa Fairweather, Marina Afanasyeva, and Noel R. Rose Animal Models of Insulin-Dependent Diabetes Edwin Liu, Liping Yu, Hiroaki Moriyama, and George S. Eisenbarth Generation, Maintenance, and Adoptive Transfer of Diabetogenic T-Cell Lines/Clones From the Nonobese Diabetic Mouse Martha J. Milton, Michelle Poulin, Clayton Mathews, and Jon D. Piganelli Experimental Use of Murine Lupus Models Stanford L. Peng The Anti-DNA Knock-In Model of Systemic Autoimmunity Induced by the Chronic Graft-vs-Host Reaction Robert Eisenberg and Arpita Choudhury Murine Models of Lupus Induced by Hypomethylated T Cells Bruce Richardson, Donna Ray, and Raymond Yung The Mouse Model of Collagen-Induced Arthritis David D. Brand, Andrew H. Kang, and Edward F. Rosloniec Proteoglycan Aggrecan-Induced Arthritis: A Murine Autoimmune Model of Rheumatoid Arthritis Tibor T. Glant and Katalin Mikecz Mouse Models of Multiple Sclerosis: Experimental Autoimmune Encephalomyelitis and Theiler's Virus-Induced Demyelinating Disease Kevin G. Fuller, Julie K. Olson, Laurence M. Howard, J. Ludovic Croxford, and Stephen D. Miller Experimental Autoimmune Encephalomyelitis Praveen Rao and Benjamin M. Segal Animal Models of Scleroderma Gabriella Lakos, Shinsuke Takagawa, and John Varga Rodent Models of Experimental Autoimmune Uveitis Rajeev K. Agarwal and Rachel R. Caspi Autoimmune Depigmentation Following Sensitization to Melanoma Antigens Arthur A. Hurwitz and Qingyong Ji Indexshow more