Atomic Force Microscopy

Atomic Force Microscopy : Biomedical Methods and Applications

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The natural, biological, medical, and related sciences would not be what they are today without the microscope. After the introduction of the optical microscope, a second breakthrough in morphostructural surface analysis occurred in the 1940s with the development of the scanning electron microscope (SEM), which, instead of light (i. e. , photons) and glass lenses, uses electrons and electromagnetic lenses (magnetic coils). Optical and scanning (or transmission) electron microscopes are called "far-field microscopes" because of the long distance between the sample and the point at which the image is obtained in comparison with the wavelengths of the photons or electrons involved. In this case, the image is a diffraction pattern and its resolution is wavelength limited. In 1986, a completely new type of microscopy was proposed, which, without the use of lenses, photons, or electrons, directly explores the sample surface by means of mechanical scanning, thus opening up unexpected possibilities for the morphostructural and mechanical analysis of biological specimens. These new scanning probe microscopes are based on the concept of near-field microscopy, which overcomes the problem of the limited diffraction-related resolution inherent in conventional microscopes. Located in the immediate vicinity of the sample itself (usually within a few nanometers), the probe records the intensity, rather than the interference signal, thus significantly improving resolution. Since the most we- known microscopes of this type operate using atomic forces, they are frequently referred to as atomic force microscopes (AFMs).show more

Product details

  • Hardback | 394 pages
  • 157.5 x 231.1 x 27.9mm | 884.52g
  • Humana Press Inc.
  • Totowa, NJ, United States
  • English
  • 242nd 2004 ed.
  • XIV, 394 p.
  • 1588290948
  • 9781588290946

Back cover copy

Although atomic force microscopy (AFM) offers many significant advantages over the conventional microscopies used in the biological and medical sciences, its use is more familiar to physicists and engineers than to biomedical researchers. In Atomic Force Microscopy: Biomedical Methods and Applications, highly experienced physicians and biologists clearly explain the basic technical knowledge needed to use AFM and demonstrate its multifarious uses in biomedicine and the life sciences. The applications range widely from morphostructural analyses of cellular structures, to the investigation of subcellular structures, to functional investigations, and reveal a powerful new way of looking at biological samples. Each tested protocol includes step-by-step instructions to ensure successful experimental results, background material on the principle behind the technique, tips on troubleshooting and avoiding known pitfalls, and notes on how to distinguish artifacts from useful data. The methods clearly demonstrate the advantages of AFM over traditional life science microscopy, among them simultaneous very high magnification and resolution, minimal tissue and cell preparation, and the ability to obtain different views of the sample from a single data collection. Cutting-edge and highly practical, Atomic Force Microscopy: Biomedical Methods and Applications will help all investigators in biology and medicine open a new microscopic world, develop novel applications, and apply this powerful technology productively in their own more

Table of contents

Part I. The Basics of Atomic Force Microscopy How the Atomic Force Microscope Works Davide Ricci and Pier Carlo Braga Imaging Methods in Atomic Force Microscopy Davide Ricci and Pier Carlo Braga Recognizing and Avoiding Artifacts in AFM Imaging Davide Ricci and Pier Carlo Braga Advanced Biosensing Using Micromechanical Cantilever Arrays Martin Hegner and Youri Arntz Part II. Morphostructural Analysis of Cellular Structures Analysis of Human Fibroblasts by Atomic Force Microscopy Gillian R. Bushell, Colm Cahill, Sverre Myhra, and Gregory S. Watson Corneal Tissue Observed by Atomic Force Microscopy Stylliani Lydataki, Miltiadis K. Tsilimbaris, Eric S. Lesniewska, Alain Bron, and Iannis G. Pallikaris AFM Study of Surface Structure Changes in Mouse Spermatozoa Associated With Maturation Hiroko Takano and Kazuhiro Abe Calculation of Cuticle Step Heights from AFM Images of Outer Surfaces of Human Hair James R. Smith Imaging Living Chondrocyte Surface Structures With AFM Contact Mode Gerlinde Bischoff, Anke Bernstein, David Wohlrab, and Hans-Joachim Hein Growth Cones of Living Neurons Probed by Atomic Force Microscopy Davide Ricci, Massimo Grattarola, and Mariateresa Tedesco Evaluating Demineralization and Mechanical Properties of Human Dentin With AFM Grayson W. Marshall, Jr., Sally J. Marshall, Mehdi Balooch, and John H. Kinney Applying Atomic Force Microscopy to Studies in Cardiac Physiology Jason J. Davis, Trevor Powell, and H. Allen O. Hill Imaging Bacterial Shape, Surface, and Appendages Before and After Treatments With Antibiotics Pier Carlo Braga and Davide Ricci Part III. Subcellular Structures Investigation Visualizing Nuclear Structure In Situ by Atomic Force Microscopy Luis Felipe Jimenez-Garcia and Maria de Lourdes Segura-Valdez Imaging Surface andSubmembranous Structures in Living Cells With the Atomic Force Microscope: Notes and Tricks Filip Braet and Eddie Wisse Atomic Force Microscopy of Protein Complexes Olga I. Kiselyova and Igor V. Yaminsky Atomic Force Microscopy of Interfacial Monomolecular Films of Pulmonary Surfactant Kaushik Nag, Robert R. Harbottle, Amiyo K. Panda, and Nils O. Petersen High-Resolution Analysis of the 3D Organization of Human Metaphase Chromosomes Stefan Thalhammer, Pietro Gobbi, Mirella Falconi, Giovanni Mazzotti, and Wolfgang M. Heckl Shape and Volume of Living Aldosterone-Sensitive Cells Imaged With the Atomic Force Microscope Stefan W. Schneider, Rainer Matzke, Manfred Radmacher, and Hans Oberleithner Localization of Epithelial Sodium Channels by Atomic Force Microscopy Peter R. Smith and Dale J. Benos High-Resolution Imaging of Bacteriorhodopsin by Atomic Force Microscopy Dimitrios Fotiadis and Andreas Engel Part IV. Functional Investigations With AFM Measurement of Mechanical Properties of Intact Endothelial Cells in Fresh Arteries Hiroshi Miyazaki and Kozaburo Hayashi Observation of Oxidative Stress on Yeast Cells Ricardo de Souza Pereira Lymphoblastoid Cells Exposed to Low-Frequency Magnetic Fields: Study by Atomic Force Microscopy Settimio Grimaldi, Marco Girasole, and Antonio Cricenti Sample Preparation Method for Observing RNA Polymerase Activity by Atomic Force Microscopy Sandor Kasas Atomic Force Microscopy of b-Amyloid: Static and Dynamic Studies of Nanostructure and Its Formation Justin Legleiter and Tomasz Kowalewski How to Build Up Biosensors With the Cantilever of the Atomic Force Microscope Ricardo de Souza Pereira Measurement of Single Molecular Interactions by Dynamic Force Microscopy Martin Hegner, Wilfried Grange, and Patricia Bertoncini Indexshow more

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