Animal Cell Culture Methods

Animal Cell Culture Methods

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This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques.
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Product details

  • Paperback | 450 pages
  • 201 x 230 x 24mm | 663g
  • Academic Press Inc
  • San Diego, United States
  • English
  • New edition
  • New edition
  • b&w illustrations
  • 0124800408
  • 9780124800403

Table of contents

Principles of cell culture: Animal cell culture equipment and techniques, D. Barnes; Making informed choices - medium, serum, and serum-free medium - how to choose the appropriate medium and culture system for the model you wish to create, J.P. Mather; Cell line availability - where to get the cell lines you need, R.J. Hay; Cell culture contamination - sources, consequences, prevention, and elimination, M.G. Gabridge. Establishing cell lines: Immortalization by gene transfection, Y. Katakura et al; Establishment of mammalian testicular cell lines, M.-C. Hoffmann, J.L. Millan; Cell hybridization, hybridomas, and human hybridomas, S. Shirahata et al; establishing human glioma-derived cell lines, M. Westphal, H. Meissner; Culture methods for selective growth of normal rat and human Schwann cells, R. Li; Invertebrate cell culture considerations - insects, ticks, shellfish, and worms, C.J. Bayne; Cell line characterization and authentication, J. Kaplan, B. Hukku. Specialized culture techniques: Laboratory scale-up of cell cultures (0,5-50 litres), J.P. Mather; Cell synchronization, G.F. Merrill; Measurement of cell death, D.T. Loo, J.R. Tillema; Simultaneous measurement of cell cycle and apoptotic cell death, A. Moore et al; Embryonic stem cells, creating transgenic animals, M. Pirity et al. Microscopy and morphology: Electron microscopy - use of transmission and scanning electron microscopy to study cells in culture, D.M. Phillips; Indirect immuno-fluorescence microscopy in cultured cells, S.P. Wheatly, Y.-L.Wang; Cellular localization of mRNA and protein - in situ hybridization histochemistry and in situ ligand binding, T.K. Woodruff.
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About Jennie P. Mather

Jennie P. Mather, Residence: San Francisco, California. Affiliation: Genentech, Inc., San Francisco, California. - David Barnes, Residence: Corvallis, Oregon. Affiliation: Oregon State University, Corvallis, Oregon.
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Review quote

"...investigators with different levels of expertise will find smething of interest in many of the topics covered.. -ANALYTICAL BIOCHEMISTRY This is a useful and well written book... This volume is more complete than many others since it features a much broader coverage of important information and is more current than most others. It would be a very useful reference in any life science library or a laboratory that is using or plans to use animal cell culture. -DOODY'S
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