Analysis of Non-mammalian Genomes : A Practical Guide
Offering detailed protocols for those needing to contruct a variety of maps and isolate genes, this book is intended to popularize the new techniques of genome analysis derived from the Human Genome Project. The power of these new methods is often most striking when applied to problems outside of human genetics, particularly the nonmammalian systems on which many researchers focus. Many of these organisms are economically important and biologically rich. "Analysis of Non-Mammalian Genomes" covers the "how to" aspects of preparation, handling, cloning, and analysis of large DNA and the creation of chromosome and genome maps. This lab manual facilitates the transfer of these technologies to small "low tech" environments and allows them to be used by those with no background in genome mapping or large-fragment cloning.
- Hardback | 384 pages
- 149.86 x 228.6 x 21.08mm | 616.88g
- 25 Sep 1996
- Elsevier Science Publishing Co Inc
- Academic Press Inc
- San Diego, United States
Table of contents
Introduction to pulsed field gels, preparation, and analysis of large DNA, J.S. Lee, B. Birren, and E. Lai; electrophoretic karyotyping in fungi, K. Dewar, L. Bernier, and R. Levesque; isolation and analysis of high molecular weight DNA from plants, M. Ganal; generating and using DNA markers in plants, J.A. Rafalski, M. Morgante, W. Powell, J.M. Vogel, and S.V. Tingey; genome mapping of protozoan parasites by linking clones, S. Morzaria; mapping and analysis of bacterial genomes, U. Romling, R. Fislage, and B. Tummler; cosmid cloning with small genomes, R. Wenzal and R. Herrmann; construction of P1 artificial chromosome (PAC) libraries from lower vertebrates, C.T. Amemiya, T. Ota, and G.W. Litman; the selection of chromosome-specific DNA clones from African trypanosome genomic libraries, S. Melville, N. Shepherd, C. Gerrard, and R. Le Page; analysis of the dictyostelium discoideum genome, A. Kuspa and W. Loomis; integrated genome mapping by hybridization techniques, J.D. Hoheisel, E. Maier, R. Mott and H. Lehrach.