Excerpt from The American Practitioner and News, 1895: A Bi-Weekly Journal of Medicine and Surgery; Volumes XIX and XX
A horse, after receiving injections at intervals of from one to four weeks, becomes only very gradually immune, and it is not until the end Of from four to six months that the serum has gained any considerable antitoxic power. The amount and strength Of the virus are gradually increased as the immunizing process proceeds, so that finally an animal is able to bear several liters of undiluted toxine solution. It is to be noted, however, that the antitoxic value of the blood-serum of the immunized horse bears no definite relation to the amount of toxine that has been introduced into his. System. The antitoxic strength of the serum of the horse has to be tested from time to time by experiments on guinea-pigs, the serum being tried not too soon after an injection of the virus. When the desired antitoxic strength has been attained, from one to three liters of blood are drawn through a canula from the jugular vein into sterilized vessels, and cooled in the ice-box for from twelve to fourteen hours. The serum is then poured off, and after per cent carbolic acid has been added its exact antitoxic strength is tested upon animals. After the blood has been extracted the animal is usually hungry and thirsty. If it bears the blood-letting well the process may be repeated two or three times in the course of eight days, after which there must be a renewal of the vaccinations for several weeks.
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