ABC Transporters: Biochemical, Cellular, and Molecular Aspects: Volume 292

ABC Transporters: Biochemical, Cellular, and Molecular Aspects: Volume 292

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 285 volumes have been published (all of them still in print) and much of the material is relevant even today-truly an essential publication for researchersin all fields of life sciences.
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Product details

  • Hardback | 853 pages
  • 154 x 228 x 42mm | 1,360.79g
  • Academic Press Inc
  • San Diego, United States
  • English
  • 0121821935
  • 9780121821937

Table of contents

Prokaryotic ABC Transporters: Overview of Bacterial ABC Transporters Preparation and Reconstitution of Membrane-Associated Maltose Transporter Complex of Escherichia coli. Maltose Transport in Escherichia coli: Mutations That Uncouple ATP Hydrolysis from Transport Binding Protein-Dependent ABC Transport System for Glycerol 3-Phosphate of Escherichia coli.

Eukaryotic ABC Transporters: Cloning of Novel ABC Transporter Genes ABC Proteins and Their Relevance to Human Health and Disease.

A. Nonmammalian ABC Transport Systems: MDR Genes: Molecular Cloning and Protein Chemical Aspects Functional Analysis of pfmdr1 Gene of Plasmodium falciparum Amplification of ABC Transporter Gene pgpA and of Other Heavy Metal Resistance Genes in Leishmania tarentolae and Their Study by Gene Transfection and Gene Disruption Functional Assay for Analysis of Yeast ste6 Mutants ABC Transporters Involved in Transport of Eye Pigment Precursors in Drosophila melanogaster.

B. Mammalian P-Glycoproteins: Isolation of Altered Function Mutants and Genetic Supressor Elements of Multidrug Transporter P-Glycoprotein by Expression Selection from Retroviral Libraries Selection and Maintenance of Multidrug-Resistant Cells Monoclonal Antibodies to P-Glycoprotein: Preparation and Applications to Basic and Clinical Research Topology of P-Glycoproteins Use of Cell-Free System to Determine P-Glycoprotein Transmembrane Topology Photoaffinity Labels for Characterizing Drug Interaction Sites of P-Glycoprotein Identification of Drug Interaction Sites in P-Glycoprotein Photoaffinity Labeling of Human P-Glycoprotein: Effect of Modular Interaction and ATP Hydrolysis on Substrate Binding Identification of Phosphorylation Sites in Human MDR1 P-Glycoprotein Identification of In Vivo Phosphorylation Sites for Basic-Directed Kinases in Murine mdr1b P-Glycoprotein by Combination of Mass Spectrometry and Site-Directed Mutagenesis P-Glycoprotein and Swelling-Activated Chloride Channels Functional Expression of mdr and mdr-like cDNAs in Escherichia coli Yeast Secretory Vesicle System for Expression and Functional Characterization of P-Glycoproteins High Level Expression of Mouse Mdr3 P-Glycoprotein in Yeast Pichia pastoris and Characterization of ATPase Activity Mutational Analysis of P-Glycoprotein in Yeast Saccaromyces cerevisiae Baculovirus-Mediated Expression of Human Multidrug Resistance cDNA in Insect Cells and Functional Analysis of Recombinant P-Glycoprotein Recombinant Vaccinia Virus Vectors for Functional Expression of P-Glycoprotein in Mammalian Cells Functional Expression of Human P-Glycoprotein from Plasmids Using a Vaccinia Virus-Bacteriophage T7 RNA Polymerase System A pHaMDR-DHFR Bicistronic Expression System for Mutational Analysis of P-Glycoprotein Mutational Analysis of Human P-Glycoprotein Purification and Reconstruction of Human P-Glycoprotein Drug-Stimulatable ATPase Activity in Crude Membranes of Human MDR1-Transfected Mammalian Cells ATPase Activity of Chinese Hamster P-Glycoprotein Construction of MDR1 Vectors for Gene Therapy Construction of MDR1 Adeno-Associated Virus for Gene Therapy Retroviral Transfer of the Human MDR 1 into Human T-Lymphocytes Construction and Analysis of Multidrug Resistance Transgenic Mice.

C. Multidrug Resistance Associated Protein: Cloning, Transfer, and Characterization of Multidrug Resistance Protein Transport Function and Substrate Specificity of Multidrug Resistance Protein.

D. Cystic Fibrosis Transmembrane Conductance Regulator: Heterologous Expression Systems for Study of Cystic Fibrosis Transmembrane Conductance Regulator Characterization of Polyclonal and Monoclonal Antibodies to Cystic Fibrosis Transmembrane Conductance Regulator Identification of Cystic Fibrosis Transmembrane Conductance Regulator in Renal Endosomes Assays of Dynamics, Mechanisms, and Regulation of ATP Transport and Release: Implications for Study of ABC Transporter Function Overexpression, Purification, and Function of First Nucleotide Binding Fold of Cystic Fibrosis Transmembrane Conductance Regulator Recombinant Synthesis of Cystic Fibrosis Transmembrane Conductance Regulator and Functional Nucleotide Binding Domains Cationic Lipid Formulations for Intracellular Gene Delivery of Cystic Fibrosis Transmembrane Conductance Regulator to Airway Epithelia Adeno-Associated Virus Vectors for Gene Therapy of Cystic Fibrosis.

E. Sulfonylurea Receptor: Sulfonylurea Receptors and ATP-Sensitive Potassium Ion Channels

F. Intracellular ABC Transporters: Peptide Transport Assay for TAP Function Peroxisomal ABC Transporters Mitochondrial ABC Transporters
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Praise for the Volume "...the book describes techniques so diverse that they include all major laboratory approaches of modem biochemistry, cell biology, and molecular biology. It provides step-by-step protocols and overviews in the field that also allow newcomers to obtain an orientation over a wide spectrum of aspects... The content is very carefully edited for clarity and precision of the described procedures. Anyone in possession of the equipment needed will be able to reproduce the described conditions and methods." --DOODY'S PUBLISHING REVIEWSPraise for the Series "The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

"Incomparably useful." --ANALYTICAL BIOCHEMISTRY

"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." --BIO/TECHNOLOGY

"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY

"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." --AMERICAN SOCIETY OF MICROBIOLOGY NEWS

"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." --ENZYMOLOGIA

"A series that has established itself as a definitive reference for biochemists." --JOURNAL OF CHROMATOGRAPHY
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