Short Protocols in Molecular Biology

Short Protocols in Molecular Biology


Edited by Frederick M. Ausubel, Edited by Roger Brent, Edited by Robert E. Kingston, Edited by David D. Moore, Edited by J.G. Seidman, Edited by John A. Smith, Edited by Kevin Struhl

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  • Publisher: John Wiley & Sons Inc
  • Format: Hardback | 834 pages
  • Dimensions: 203mm x 251mm x 46mm | 1,451g
  • Publication date: 24 June 1992
  • Publication City/Country: New York
  • ISBN 10: 0471577359
  • ISBN 13: 9780471577355
  • Edition: 2, Revised
  • Edition statement: 2nd Revised edition

Product description

Presents shortened versions of the methods published in "Current Protocols in Molecular Biology" (CPMB). Includes step-by-step descriptions of methods covered in the first ten chapters of CPMB and provides sufficient detail to perform experiments. Cross-referenced to CPMB. Explains the hows and whys of many steps. Provides tips on safety, storage and anticipated results. Includes references and recipes for all reagents and media.

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Table of contents

Escherichia Coli, Plasmids, and Bacteriophages; Preparation and Analysis of DNA; Enzymatic Manipulation of DNA and RNA; Preparation and Analysis of RNA; Construction of Recombinant DNA Libraries; Screening Recombinant DNA Libraries; DNA Sequencing; Mutagenesis of Cloned DNA; Introduction of DNA Into Mammalian Cells; Analysis of Proteins; Immunology; DNA-Protein Interactions; Saccharomyces Cerevisiae; In Situ Hybridization and Immunohistochemistry; The Polymerase Chain Reaction; Protein Expression; Appendices; Index.