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    Guide to Protein Purification (Methods in Enzymology) (Paperback) Edited by Richard R. Burgess, Edited by Murray P. Deutscher

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    DescriptionThe 2e of this classic "Guide to Protein Purification" provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This 'Guide to' gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while it presents a comprehensive overview of the field today. It gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines including biochemistry, genetics, oncology, pharmacology, dermatology and immunology. It assembles chapters on both common and less-common relevant techniques, and provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process.


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  • Full bibliographic data for Guide to Protein Purification

    Title
    Guide to Protein Purification
    Authors and contributors
    Edited by Richard R. Burgess, Edited by Murray P. Deutscher
    Physical properties
    Format: Paperback
    Number of pages: 912
    Width: 152 mm
    Height: 221 mm
    Thickness: 38 mm
    Weight: 1,179 g
    Language
    English
    ISBN
    ISBN 13: 9780123749789
    ISBN 10: 0123749786
    Classifications

    B&T Book Type: NF
    BIC E4L: SCI
    Nielsen BookScan Product Class 3: S7.1
    B&T Modifier: Region of Publication: 01
    Ingram Subject Code: SE
    Libri: I-SE
    B&T Merchandise Category: SCI
    B&T General Subject: 710
    Warengruppen-Systematik des deutschen Buchhandels: 26760
    BISAC V2.8: SCI029000
    B&T Modifier: Academic Level: 02
    BISAC V2.8: MED044000, SCI049000
    Abridged Dewey: 572
    LC classification: QP
    BISAC V2.8: SCI010000, MED071000, SCI017000
    DC22: 547.7
    BIC subject category V2: PSBC
    BISAC V2.8: SCI007000
    DC22: 572.6
    BIC subject category V2: PSAG
    Thema V1.0: PSAK, TCB, PSF, PSD, PSB, MKG, MJCM
    Edition
    2, Revised
    Edition statement
    2nd Revised edition
    Illustrations note
    black & white illustrations, black & white tables, figures, black & white plates, colour plates
    Publisher
    Elsevier Science Publishing Co Inc
    Imprint name
    Academic Press Inc
    Publication date
    01 December 2009
    Publication City/Country
    San Diego
    Table of contents
    Why Purify Enzymes?Strategies and Considerations for Protein Purifications (Linn)Bioinformatics in Planning a Protein Purification (Burgess)Preparing a Protein Purification Summary Table (Burgess)Setting up a Laboratory (Deutscher)Buffers: Principle and Practice (Blanchard)Measurement of Enzyme Activity (Harris)Quantitation of Protein (Bailey and Noble)Maintaining Protein Stability (Deutscher)Strategy for Choosing and Expression System (Brondyk)Bacterial Expression Systems (Collart)Yeast Expression Systems (Cregg)Baculovirus-Insect Cell Expression Systems (Jarvis)Mammalian Expression Systems (Geisse and Knopf)Expression of Tagged Proteins (Melhorta)Re-folding of Solubilized Inclusion Body Proetin (Burgess)Preparation of Biological Extract (Grabski)