Culture of Human Tumor Cells

Culture of Human Tumor Cells

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Description

This comprehensive resource provides stepwise protocols for the in vitro cultivation of the major types of human tumors. Written in a reader-friendly style, this book is organized by specific tumor, discussing cultures for lung, gastric, colorectal, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. Detailed techniques for initiation, propagation, and characterization of established cell lines are presented. Drug treatment, selection, differentiation, assays for malignant cells, risks, and applications are discussed. An extensive listing of vendors for equipment and other cell culture products is also included.show more

Product details

  • Paperback | 452 pages
  • 178 x 250 x 24mm | 821g
  • John Wiley and Sons Ltd
  • John Wiley & Sons Inc
  • New York, United States
  • English
  • New.
  • colour illustrations
  • 0471438537
  • 9780471438533

Back cover copy

Human cell culture in experimental cancer research is taking on new importance in the post-genomic era, as new methods for genome-wide analysis allow researchers to analyze the effects of altered genomes on the cell. Roswitha Pfragner and R. Ian Freshney's critical compilation introduces culture of human tumor cells, from basic principles to detailed protocols, to the seasoned researcher and novice alike. Chapters are organized by tumors of clinical significance, and offer informative overviews of pathology, preparation, troubleshooting, applications, alternative methods, and a list of suppliers. This wide-ranging text covers culture for lung tumors, gastric cancer, colorectal cancer, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. In addition, Culture of Human Tumor Cells: Provides stepwise protocols by recognized experts Presents detailed techniques for initiation, propagation, and characterization of tumor cell lines Includes extensive listing of vendors for equipment and other cell culture products Discusses drug treatment, selection, differentiation, assays for malignant cells, risks, and applications Offers safety precautions, helpful tips, and a list of abbreviations Provides specific, practical details on how to prepare media and reagents for the isolation of culture protocols Scientists, research clinicians, students, and technicians in pathology, cancer biology, cell biology, biochemistry, biotechnology, and medicine will find Culture of Human Tumor Cells to be an incomparable resource.show more

Review quote

"This book should be used as reference source for culturing different tumor types. It is a cookbook of sorts, but instead of recipes it has protocols." (E-STREAMS, August 2004)show more

Table of contents

Preface.Contributors.List of Abbreviations.1. Growth of Human Lung Tumor Cells in Culture (Reen Wu).2. Culture of Normal and Malignant Gastric Epithelium (Jae-Gahb Park, Ja-Lok Ku, Hee-Sung Kim, So-Yeon Park and Michael J. Rutten).3. Establishment of Cell Lines from Colon Carcinoma (Robert H. Whitehead).4. Pancreatic Cancer-Derived Cultured Cells: Genetic Alterations and Application to an Experimental Model of Pancreatic Cancer Metastasis (Haruo Iguchi, Kazuhiro Mizumoto, Masaki Shono, Akira Kono and Soichi Takiguchi).5. The Establishment and Characterization of Bladder Cancer Cultures In Vitro (Vivian X. Fu, Steven R. Schwarze, Catherine A. Reznikoff and David F. Jarrard).6. Long-term Culture of Normal and Malignant Human Prostate Epithelial Cells (Robert K. Bright and Jennifer D. Lewis).7. The Development of Human Ovarian Epithelial Tumor Cell Lines from Solid Tumors and Ascites (Anne P. Wilson).8. Culture of Cervical Carcinoma Tumor Cell Lines (Peter Stern, Catherine West and Deborah Burt).9. Primary Culture of Human Mammary Tumor Cells (Valerie Speirs).10. Myoepithelium: Methods of Culture and Study (Sanford H. Barsky and Mary L. Alpaugh).11. Multistage Head and Neck Squamous Cell Carcinoma (Kirsten G. Edington, Isabella J. Berry, Margaret O'Prey, Julie E. Burns, Louise J. Clark, Roy Mitchell, Gerry Robertson, David Soutar, Lesley W. Coggins and E. Kenneth Parkinson).12. Culture of Melanocytes from Normal, Benign, and Malignant Lesions (Ruth Halaban).13. Establishment and Culture of Human Leukemia-Lymphoma Cell Lines (Hans G. Drexler).14. In Vitro Culture of Malignant Brain Tumors (John L. Darling).15. Culture of Human Neuroendocrine Tumor Cells (Roswitha Pfragner, Annemarie Behmel, Elisabeth Ingolic and Gerhard H. Wirnsberger).Suppliers).Index.show more

About Roswitha Pfragner

R. Ian Freshney gained a B.Sc. (1960) and Ph.D. (1964) from Glasgow University, where he is on the faculty of Medical Oncology as a senior lecturer and an Honorary Senior Research Fellow. Dr. Freshney currently teaches a number of international basic and specialized cell culture courses, and his research interests include human tumor culture. He is the author of Culture of Animal Cells, a Manual of Basic Technique, and has edited numerous other books on specialized cell culture. Roswitha Pfragner, Ph.D., studied biology at the University of Vienna, Austria and is currently a senior lecturer at the Institute of Pathophysiology. Her research interests include human neuroendocrine tumors, multiple endocrine neoplasia, drug resistance, apoptosis, and genetic events in tumorigenesis.show more

Review Text

"This book should be used as reference source for culturing different tumor types. It is a cookbook of sorts, but instead of recipes it has protocols." ( E-STREAMS , August 2004)show more