Culture of Animal Cells

Culture of Animal Cells : A Manual of Basic Technique

  • Other digital
By (author) R. Ian Freshney

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This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since the publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded "Fifth Edition of Culture of Animal Cells". In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced. The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes: an all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols; and new coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering. It also includes: an expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment; enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text; fully updated references; the clearest, most consistent presentation of step-by-step protocols available; numerous diagrams, photographs, tables, and charts; and detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with web sites. Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.

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  • Other digital
  • 26 Oct 2005
  • John Wiley and Sons Ltd
  • John Wiley & Sons Inc
  • New York
  • 5th
  • 0471747599
  • 9780471747598

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Author Information

R. IAN FRESHNEY, PhD, is an honorary senior research fellow in the Centre for Oncology and Applied Pharmacology, part of the Cancer Research UK Beatson Laboratories at the University of Glasgow. He is the author or editor of numerous books and a world--renowned expert on cell culture technique.

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Review quote

."..a good user friendly resource...a handy reference book for routing cell culture protocols." ("In Vitro Cellular & Development Biology - Animal, " May/June 2006)

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