• Culture of Animal Cells: A Manual of Basic Technique

    Culture of Animal Cells: A Manual of Basic Technique (Other digital) By (author) R. Ian Freshney

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    DescriptionThis is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since the publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded "Fifth Edition of Culture of Animal Cells". In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced. The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes: an all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols; and new coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering. It also includes: an expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment; enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text; fully updated references; the clearest, most consistent presentation of step-by-step protocols available; numerous diagrams, photographs, tables, and charts; and detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with web sites. Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.

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  • Full bibliographic data for Culture of Animal Cells

    Culture of Animal Cells
    A Manual of Basic Technique
    Authors and contributors
    By (author) R. Ian Freshney
    Physical properties
    Format: Other digital
    Width: 200 mm
    Height: 250 mm
    Weight: 666 g
    ISBN 13: 9780471747598
    ISBN 10: 0471747599

    BIC E4L: SCI
    BIC subject category V2: PS, PN
    Nielsen BookScan Product Class 3: S7.5
    BIC subject category V2: TCB
    BISAC V2.8: SCI017000
    Thema V1.0: PN, PS, TCB
    Edition statement
    John Wiley and Sons Ltd
    Imprint name
    John Wiley & Sons Inc
    Publication date
    26 October 2005
    Publication City/Country
    New York
    Author Information
    R. IAN FRESHNEY, PhD, is an honorary senior research fellow in the Centre for Oncology and Applied Pharmacology, part of the Cancer Research UK Beatson Laboratories at the University of Glasgow. He is the author or editor of numerous books and a world--renowned expert on cell culture technique.
    Review quote
    ."..a good user friendly resource...a handy reference book for routing cell culture protocols." ("In Vitro Cellular & Development Biology - Animal, " May/June 2006)
    Table of contents
    List of Figures. List of Color Plates. Preface. Abbreviations. 1. Introduction. 2. Training Programs. 3. Biology of Cultured Cells. 4. Laboratory Design and Layout. 5. Equipment. 6. Aseptic Technique. 7. Safety, Bioethics, and Validation. 8. Culture Vessels and Substrates. 9. Defined Media and Supplements. 10. Serum--Free Media. 11. Preparation and Sterilization. 12. Primary Culture. 13. Subculture and Cell Lines. 14. Cloning and Selection. 15. Cell Separation. 16. Characterization. 17. Differentiation. 18. Transformation and Immortalization. 19. Contamination. 20. Cryopreservation. 21. Quantitation. 22. Cytotoxocity. 23. Culture of Specific Cell Types. 24. Culture of Tumor Cells. 25. Organotypic Culture. 26. Scale--Up. 27. Specialized Techniques. 28. Problem Solving. 29. In Conclusion. Appendix I: Preparation of Reagents. Appendix II: Sources of Equipment and Materials. Appendix III: Suppliers and Other Resources. Appendix IV: Glossary. Appendix V: General Textbooks and Relevant Journals. References. Index.