• Culture of Animal Cells: A Manual of Basic Technique

    Culture of Animal Cells: A Manual of Basic Technique (Other digital) By (author) R. Ian Freshney

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    DescriptionThis is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of "Culture of Animal Cells". In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced. The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes: an all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering; an expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment; enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text; and fully updated references. It includes: the clearest, most consistent presentation of step-by-step protocols available; numerous diagrams, photographs, tables, and charts; and detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites. Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.


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  • Full bibliographic data for Culture of Animal Cells

    Title
    Culture of Animal Cells
    Subtitle
    A Manual of Basic Technique
    Authors and contributors
    By (author) R. Ian Freshney
    Physical properties
    Format: Other digital
    Number of pages: 672
    ISBN
    ISBN 13: 9780471739913
    ISBN 10: 047173991X
    Classifications

    BIC E4L: SCI
    BIC subject category V2: PS, PN
    Nielsen BookScan Product Class 3: S7.5
    BISAC V2.8: SCI017000
    Thema V1.0: PN, PS
    Edition
    5
    Edition statement
    5th
    Publisher
    John Wiley and Sons Ltd
    Imprint name
    John Wiley & Sons Inc
    Publication date
    15 November 2005
    Publication City/Country
    New York