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    Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part A: Part A (Methods in Enzymology) (Hardback) Edited by Daniel Herschlag

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    DescriptionThis MIE volume provides laboratory techniques that aim to predict the structure of a protein which can have tremendous implications ranging from drug design, to cellular pathways and their dynamics, to viral entry into cells. Expert researchers introduce the most advanced technologies and techniques in protein structure and folding. This title includes techniques on tiling assays.


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  • Full bibliographic data for Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part A

    Title
    Biophysical, Chemical, and Functional Probes of RNA Structure, Interactions and Folding: Part A
    Subtitle
    Part A
    Authors and contributors
    Edited by Daniel Herschlag
    Physical properties
    Format: Hardback
    Number of pages: 496
    Width: 155 mm
    Height: 231 mm
    Thickness: 28 mm
    Weight: 885 g
    Language
    English
    ISBN
    ISBN 13: 9780123743992
    ISBN 10: 0123743990
    Classifications

    B&T Book Type: NF
    BIC E4L: SCI
    Nielsen BookScan Product Class 3: S7.1
    B&T Modifier: Region of Publication: 01
    BIC subject category V2: PSAK
    Ingram Subject Code: SE
    Libri: I-SE
    B&T General Subject: 710
    B&T Modifier: Academic Level: 02
    B&T Merchandise Category: POD
    BISAC V2.8: SCI049000
    Abridged Dewey: 572
    LC classification: QP
    Warengruppen-Systematik des deutschen Buchhandels: 16760
    BISAC V2.8: SCI007000
    DC22: 572.633, 572.88
    BIC subject category V2: PSAG
    Thema V1.0: PSAK, PSD
    Edition
    1
    Illustrations note
    Illustrations (some col.)
    Publisher
    Elsevier Science Publishing Co Inc
    Imprint name
    Academic Press Inc
    Publication date
    01 December 2009
    Publication City/Country
    San Diego
    Table of contents
    A. Chemical and Enzymatic Footprinting of RNA Structure 1. Equilibrium hydroxyl radical footprinting 2. Bench-top time-resolved hydroxyl radical footprinting 3. Analysis of hydroxyl radical footprinting gels 'SAFA' 4. Kinetic modeling of reaction pathways from hydroxyl radical data 5. "BABE" mapping of protein/RNA position 6. Multiplexed..(MOHCA) 7. SHAPE 8. "In-line Probing" 9. NAIM 10. Other NAIM 11. Purification of T7 RNA Polymerase 12. Purification of T4 RNA ligase 13. In vitro transcription of RNA 14. 32P-labeling of RNA B. BIOPHYSICAL TECHNIQUES 15. Fluorescent labeling of RNAs 16. Assembly of complex RNAs by 'Moore-Sharp' ligations 17. Assembly of complex RNAs by 'Moore-Sharp' ligations 18. General considerations for smFRET with RNA samples 19. Ion counting 20. ASAXS 21. NLPB 22. smNLPB 23. Gel mobility mapping of junction structure 24. Temperature gradient gels 25. Melting studies 26. Co-transcriptional folding studies 27. Activity assays to follow folding processes 28. 2AP fluorescence 29. EPR measurements of RNA dynamics 30. EPR measurements of RNA dynamics 31. FPA measurements of RNA dynamics 32. EPR methods to study specific metal ion binding sites in RNA 33. Thermodynamic study of site-specific metal ion binding sites in RNA 34. Oligonucleotide hybridization studies of RNA folding pathways 35. Native gel shifts 36. Tiling arrays to assess RNA structure 37. EPR distance measurements in RNA 38. RNA folding in vivo 39. Cleavage of RNAs with 'restriction DNAzymes'